A review of the reproduction and rearing of clownfish, Amphiprion clarkii, in captivity

Main Article Content

Sarvi B.
Pourmozaffar S

Abstract

Clownfishes are very popular among ornamental fish keepers due to their aesthetic appeal and easy adaptability to captive conditions. The captive breeding of these fish not only meets the market demand but also prevents the destruction of their natural habitats during fishing. In Iran, Amphiprion clarkii has been successfully bred and reared in the Bandar Lengeh mollusks research center. The broodfish were fed twice a day in the morning and the afternoon at 5% of body weight. The pot was used as a shelter for broodfish spawning in the aquarium. One indication of impending spawning was the male and female broodfish cleaning inside the shelter. In this species, the number of eggs per spawning was 300-400. The eggs were sticky and protected by their parents during the incubation period. At 28 ◦C, the eggs were hatched after six days. During the first two weeks after hatching, rotifer and Artemia nauplii were exclusively utilized to feed the larvae. The weaning process started 15 days after hatching (DAH), and on 25 DAH, all the larvae completely shifted to the microparticulate diet. To improve the color of farmed fishes, the formulated feed prepared for the larvae and juveniles was supplemented with 100 ppm and 200 ppm astaxanthin, respectively. During 2-4 months, the juveniles reached the size of 3-4 cm and were ready to be sold in the market. The survival rate during this period was more than 50%.

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A review of the reproduction and rearing of clownfish, Amphiprion clarkii, in captivity (S. B. & P. S, Trans.). (2024). International Journal of Aquatic Research and Environmental Studies, 4(2), 145-158. https://doi.org/10.70102/wynp2t33

References

Chen, J.Y., Zeng, C., Jerry, D.R. and Cobcroft, J.M., 2020. Recent advances of marine ornamental fish larviculture: broodstock reproduction, live prey and feeding regimes, and comparison between demersal and pelagic spawners. Reviews in Aquaculture, 12(3), 1518-1541. [http://DOI:10.1111/raq.12394](http://DOI:10.1111/raq.12394)

Dhaneesh, K.V., Ajith Kumar, T.T., Swagat, G. and Balasubramanian, T., 2012. Breeding and mass scale rearing of clownfish Amphiprion percula: feeding and rearing in brackishwater. Chinese Journal of Oceanology and Limnology, 30, 528-534. DOI:10.1007/s00343-012-1184-x

Elbal, M.T., Hernández, M.G., Lozano, M.T. and Agulleiro, B., 2004. Development of the digestive tract of gilthead sea bream (Sparus aurata L.). Light and electron microscopic studies. Aquaculture, 234(1-4), 215-238. [https://DOI.org/10.1016/j.aquaculture.2003.11.028](https://DOI.org/10.1016/j.aquaculture.2003.11.028)

Gordon, A.K. and Hecht, T., 2002. Histological studies on the development of digestive system of the clownfish Amphiprion percula and the time of weaning. Journal of Applied Ichthyology, 18, 113-117. [http://DOI:10.1046/j.1439-0426.2002.00321.x](http://DOI:10.1046/j.1439-0426.2002.00321.x)

Green, B.S. and McCormick, M.I., 2006. Position of egg within a clutch is linked to size at hatching in a demersal tropical fish. Journal of Experimental Marine Biology and Ecology, 329, 144-152. DOI:10.1016/j.jembe.2005.08.012

Kandathil Radhakrishnan, D., AkbarAli, I., Schmidt, B.V., John, E.M., Sivanpillai, S. and Thazhakot Vasunambesan, S., 2020. Improvement of nutritional quality of live feed for aquaculture: An overview. Aquaculture Research, 51(1), 1-17. [https://DOI.org/10.1111/are.14357](https://DOI.org/10.1111/are.14357)

Kolkovski, S., 2013. Microdiets as alternatives to live feeds for fish larvae in aquaculture: Improving the efficiency of feed particle utilization. In Advances in Aquaculture Hatchery Technology (pp. 203-222). Woodhead Publishing. [https://DOI.org/10.1533/9780857097460.1.203](https://DOI.org/10.1533/9780857097460.1.203)

Kolkovski, S., Lazzo, J., Leclercq, D. and Izquierdo, M., 2009. Fish Larvae Nutrition and Diet: New Developments. In: Burnell, G. and Allan, G. (Eds.), New Technologies in Aquaculture. CRC Press, Oxford, Cambridge, New Delhi, 1163 p. [http://DOI:10.1533/9781845696474.3.315](http://DOI:10.1533/9781845696474.3.315)

Langdon, C., 2003. Microparticle types for delivering nutrients to marine fish larvae. Aquaculture, 227, 259-275. [http://DOI:10.1016/S0044-8486(03)00508-8](http://DOI:10.1016/S0044-8486%2803%2900508-8)

Lian, P., Lee, C.M. and Bengtson, D.A., 2008. Development of a squid-hydrolysate-based larval diet and its feeding performance on summer flounder, Paralichthys dentatus larvae. Journal of the World Aquaculture Society, 39(2), 196-204. [https://DOI.org/10.1111/j.1749-7345.2008.00152.x](https://DOI.org/10.1111/j.1749-7345.2008.00152.x)

Marte, C., 2003. Larviculture of marine species in Southeast Asia: current research and industry prospects. Aquaculture, 227, 293-304. [http://DOI:10.1016/S0044-8486(03)00510-6](http://DOI:10.1016/S0044-8486%2803%2900510-6)

Mutti, D.W., Ballester, E.L., Martino, R.C., Wasielesky, W. and Cavalli, R.O., 2017. Feeding n-3 HUFA enriched Artemia to the larvae of the pink shrimp Farfantepenaeus paulensis increases stress tolerance and subsequent growth. Latin American Journal of Aquatic Research, 45(1), 18-24. DOI:10.3856/vol45-issue1-fulltext-2

Olivotto, I., Cardinali, M., Barbaresi, L., Maradonna, F. and Carnevali, O., 2003. Coral reef fish breeding: the secret of each species. Aquaculture, 224, 69-78. [http://DOI:10.1016/S0044-8486(03)00207-2](http://DOI:10.1016/S0044-8486%2803%2900207-2)

Olivotto, I., Capriotti, F., Buttino, I., Avella, M.A., Vitiello, V., Maradonna, F. and Carnevali, O., 2008. The use of harpacticoid copepods as live prey for Amphiprion clarkii larviculture: Effects on larval survival and growth. Aquaculture, 274, 347-352. [http://DOI:10.1016/j.aquaculture.2007.11.027](http://DOI:10.1016/j.aquaculture.2007.11.027)

Olivotto, I., Holt, G.H. and Carnevali, O., 2009. Advances in Marine Ornamental Aquaculture: Breeding and Rearing Studies. In: Davin, T.B. and Brannet, A.P. (Eds.), Coral Reefs Biology, Threats and Restoration. Nova Science Publisher, Inc., pp. 1-43. [http://DOI:10.1111/j.1749-7345.2011.00453.x](http://DOI:10.1111/j.1749-7345.2011.00453.x)

Olivotto, I., Planas, M., Simões, N., Holt, G.J., Avella, M.A. and Calado, R., 2011. Advances in breeding and rearing marine ornamentals. Journal of the World Aquaculture Society, 42(2), 135-166. [https://DOI.org/10.1111/j.1749-7345.2011.00453.x](https://DOI.org/10.1111/j.1749-7345.2011.00453.x)

Onal, U., Langdon, C. and Celik, I., 2008. Ontogeny of the digestive tract of larvae percula clownfish, Amphiprion percula (Lacepede 1802): a histological perspective. Aquaculture Research, 39, 1077-1086. [http://DOI:10.1111/j.1365-2109.2008.01968.x](http://DOI:10.1111/j.1365-2109.2008.01968.x)

Palmtag, M.R., 2017. The marine ornamental species trade. In: Calado, R., Olivotto, I., Oliver, M.P. and Holt, G.J. (Eds.), Marine Ornamental Species Aquaculture, pp. 3-14. John Wiley & Sons Ltd., Chichester, UK. DOI:10.1002/9781119169147.ch1

Pan, Y.J., Dahms, H.U., Hwang, J.S. and Souissi, S., 2022. Recent trends in live feeds for marine larviculture: A mini review. Frontiers in Marine Science, 9, 864165. [https://DOI.org/10.3389/fmars.2022.864165](https://DOI.org/10.3389/fmars.2022.864165)

Pham, H.D., Siddik, M.A., Rahman, M.A., Huynh, L.T., Nahar, A. and Vatsos, I.N., 2023. Effects of n-3 HUFA-enriched Artemia on growth, biochemical response, skeletal morphology and stress resistance of Asian sea bass (Lates calcarifer) larvae reared at high temperature. Aquaculture, 574, 739732. [https://DOI.org/10.1016/j.aquaculture.2023.739732](https://DOI.org/10.1016/j.aquaculture.2023.739732)

Pouil, S., Tlusty, M.F., Rhyne, A.L. and Metian, M., 2020. Aquaculture of marine ornamental fish: overview of the production trends and the role of academia research in progress. Reviews in Aquaculture, 12(2), 1217-1230. [https://DOI.org/10.1111/raq.12381](https://DOI.org/10.1111/raq.12381)

Singh, P.K., Munilkumar, S., Sundaray, J.K., Santhanam, P., Sharma, A., Haque, R. and Satheesh, M., 2023. Effect of selenium, vitamin C and highly unsaturated fatty acids-enriched Brachionus calyciflorus on growth, survival, physio-metabolic and antioxidative responses in Anabas testudineus (Bloch, 1792) larvae. Aquaculture, 568, 739293. [https://DOI.org/10.1016/j.aquaculture.2023.739293](https://DOI.org/10.1016/j.aquaculture.2023.739293)

Tartila, S.S.Q., Abdillah, A.A. and Saramoutia, A., 2023. The Clownfish (Amphiprion spp.) Larviculture Technique with Recirculating Aquaculture System (RAS) in Buleleng, Bali. Journal of Aquaculture Development and Environment, 6(1), 363-369. [https://DOI.org/10.31002/jade.v6i1.7602](https://DOI.org/10.31002/jade.v6i1.7602)

Yasir, I. and Qin, J.G., 2009. Impact of background on color performance of false clownfish, Amphiprion ocellaris, Cuvier. Journal of the World Aquaculture Society, 40, 724-734. [https://DOI.org/10.1111/j.1749-7345.2009.00292.x](https://DOI.org/10.1111/j.1749-7345.2009.00292.x)

Yasir, I. and Qin, J.G., 2010. Effect of dietary carotenoids on skin color and pigments of false clownfish, Amphiprion ocellaris, Cuvier. Journal of the World Aquaculture Society, 41, 308-318. [https://DOI.org/10.1111/j.1749-7345.2010.00373.x](https://DOI.org/10.1111/j.1749-7345.2010.00373.x)

Yufera, M. and Darias, M.J., 2007. The onset of exogenous feeding in marine fish larvae. Aquaculture, 268, 53-63. [https://DOI.org/10.1016/j.aquaculture.2007.04.050](https://DOI.org/10.1016/j.aquaculture.2007.04.050)

Zambonino-Infante, J.L., Gisbert, E., Sarasquete, C., Navarro, I., Gutiérrez, J. and Cahu, C.L., 2008. Ontogeny and physiology of the digestive system of marine fish larvae. Feeding and Digestive Functions of Fishes, pp. 281-348.