Comparative analysis of mycelial growth of yanagi matsutake mushroom (agrocybe aegerita, v. Brig.) Across various indigenous culture media

Abstract

Media plays a critical role in the cultivation of mushrooms, serving as a method for storing viable cell lines of microorganisms. These cell lines can lead to significant advancements for the benefit of humanity. This study aimed to identify the optimal indigenous substrates and inoculum sizes that would promote faster mycelial growth in the preparation of culture media for the Yanagi Matsutake mushroom.  The study assessed the impact of different indigenous culture media and inoculation sizes on the mycelial growth of Yanagi Matsutake, focusing on the number of days required for complete mycelial ramification.The research employed a laboratory experimentation approach using a Factorial in Randomized Complete Block Design (RCBD) to investigate the effects of culture media and inoculum size on the mycelial growth of Agrocybe aegerita. The data collected was analyzed using a repeated measures analysis of variance (ANOVA) to determine the effect of the different culture media and inoculation size in mycelial growth. It also compared these effects to determine the most cost-effective culture media and inoculum size using Return on Expenditure as a measurement tool. Additionally, the study sought to develop a learning module for cultivating the Yanagi Matsutake mushroom using various culture media and inoculation sizes.  The findings indicate that the combination of specific indigenous culture media and inoculum sizes significantly affects the rate of mycelial growth and ramification of Yanagi Matsutake. Larger inoculum sizes (15-20 mm) generally resulted in faster mycelial growth and fewer days to full ramification, particularly with PDA and PSA media. The CGSA medium also showed a notable advantage for larger inoculum sizes (10 mm) compared to the smallest size of 5 mm.   In conclusion, the choice of culture medium and inoculum size is essential for optimizing the mycelial growth of Yanagi Matsutake. This study provides valuable insights into the factors that influence the mycelial growth of Yanagi, emphasizing the importance of specific indigenous culture media and inoculum sizes in achieving optimal growth rates.