Effect of adding Pomegranate Seed Pomace to the feed to reduce pathological histological changes in the liver of broiler chickens infected with Aspergillus niger toxins
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The study aimed to isolate the Aspergillus niger from pelleted poultry feed, and to study its production of mycotoxins in a number of regions of Karbala Province, represented by (Tuwairij, Qantara Al-Salam, Al-Tahadi neighborhood and Fariha) and to characterize it morphologically and partially during 20/9/2025. The isolation and diagnosis of the pathogen were completed based on their morphological, molecular and Sequencing revealed A. niger isolate that have been registered at the GeneBank, The world record for Iraqi isolation according to the entry numbers PX569964.1. In comparison to the control group the mycotoxin infected poultry revealed histopathological lesions particularly liver showing necrosis, vascular congestion, proliferation of inflammatory cells and mild to severe hepatocellular degenerationThe liver displayed slight hepatocellular degenerative changes after treating poultry with pomegranate seed Pomace with 2%. Administering nystatin, at a concentration of 1.25 g/kg of feed, to poultry previously treated with mycotoxins resulted in mild degenerative changes in liver cells. The Nystatin 1.25g/kg + Pomegranate Seed Pomace to poultry previously treated with mycotoxin show the highest level of histological improvement was seen in this group. The architecture was nearly typical, with little cellular deterioration and no necrosis.
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