Comparison of the effect of olanzapine and pimozide on development and changes of oocytes , endocrine marker and aromatase enzyme in Trichogaster trichoptrus
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Abstract
In this survey the effects of olanzapine and pimozide on endocrine hormones and aromatase enzyme in Trichogaster trichopterus were analyzed. For this reason, 120 mature female fishes were divided into 8 treatments and two control groups. The fishes in one control group received ethanol 70% while the other control group was intact. All injections were done in muscles between the dorsal fin and lateral line (0.02 mL per injection). Fishes were killed 7 days after the injection and their ovaries were dissected for histologic studies. The fish ovaries were fixed in formalin 10%. After tissue passage, cutting, and H and E staining, the ovary tissues in all treatment groups were studied by optical microscope and were compared with control groups. Then, endocrine hormone and aromatase levels in all treatment groups were compared with fishes in control groups by SPSS software and Duncan and one-way ANOVA tests. The differences between averages in different treatment fishes were determined with p-value ≤ 0.05. The results of comparison between control and treatment groups of 1, 2, 3, and 4 receiving 1, 3, 6, and 10 mg/kg b.w. of olanzapine respectively, and treatment groups of 5, 6, 7, and 8 receiving 1, 3, 6, and 10 mg/kg b.w. of pimozide respectively, showed the effects of dopamine antagonists on endocrine hormones and aromatase enzyme, which decreased aromatase, testosterone, and estradiol levels and thus had a significant difference compared with control fishes. Studying the oocyte histologic samples by photomicroscopy in the control group showed that cells were in vitellogenesis and maturation stages, while in pimozide and olanzapine treatments, with increasing dosages, despite the presence of cells in cortical stage, the cell number in prenuclear stage increased steadily. The results of this study showed that dopamine antagonists affect oocyte maturation.
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