Siderophore-Mediated Growth Enhancement in Arthrospira platensis
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Abstract
Iron availability is a critical factor influencing the growth and productivity of microalgae and cyanobacteria. In the present study, the effect of siderophore-producing bacteria, Pseudomonas lundensis and Advenella mimigardefordensis, and their crude siderophore extracts was evaluated on the growth of Arthrospira platensis. Cultures were grown in Zarrouk’s medium under controlled laboratory conditions and subjected to six treatment groups, including iron-limited control, EDTA supplementation, bacterial inoculation, and siderophore addition. Growth was monitored over 7 days using optical density, wet biomass, and direct microscopic count, while iron uptake was assessed using the Ferrozine assay.
Siderophore-treated cultures exhibited significantly enhanced growth compared to EDTA and control treatments, with Pseudomonas lundensis siderophore showing the highest increase in optical density, biomass, and cell density. Two-way ANOVA revealed highly significant effects (p < 0.0001) of time, treatment, and their interaction on all growth parameters. Iron uptake analysis showed a marked reduction in residual iron concentration in siderophore-treated samples (~0.5–0.6 mg/L) compared to EDTA (~1.2 mg/L) and control (~1.8 mg/L), indicating superior iron chelation and uptake efficiency. Overall, the results demonstrate that siderophores significantly enhance iron bioavailability and promote growth of A. platensis, highlighting their potential application in improving microalgal cultivation and productivity.
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