Cytotoxic and Apoptotic Potential of Scylla serrate (Mud Crab) Shell Extract Silver Nanoparticles Biosynthesized against Breast Cancer (MCF-7) Cell Lines

Abstract

Breast cancer is the most common type of cancer in women. Despite various pharmacological developments, the identification of new therapies is still required for treating breast cancer. Crab is often recommended as a traditional medicine for cancer. This study aimed to determine the in vitro effect of a hydroalcoholic crab shell extract on a breast cancer cell line. In this experimental study, MCF7 breast cancer cell line was used. Crab shell was powdered and a hydroalcoholic (70° ethanol) extract was prepared. Five concentrations (100, 200, 400, 800, and 1,000 μg/mL) were added to the cells for three periods, 24, 48, and 72 hours. Increasing the dose significantly increased apoptosis (p<0.001). NO secretion from MCF7 cells significantly decreased in response to different concentrations of the extract in a dose- and time-dependent manner (p<0.050). The crab shell extract inhibited the proliferation of MCF7 cells by increasing apoptosis, MTT assay.