Biosynthesis of Zinc Oxide Nanoparticles from the Deproteinised Leaf Juice and Antibacterial Evaluation

Abstract

Nanoparticles are established through-processing, consolidating, and deforming materials at the nanoscale. They offer benefits in terms of magnetic properties, catalytic activity, electronic properties, antimicrobial properties and anti-inflammatory effects. Green synthesis of nanoparticle is done through plants. The Medicago sativa L. is a well-known forage crop worldwide. It is rich in secondary metabolites, essential amino acids, and vitamins. This study investigated the synthesis of zinc oxide nanoparticles via a plant mediated approach. Zinc oxide nanoparticles were successfully synthesized with 0.1M zinc acetate dihydrate, reacted with the deproteinized juice (DPJ) of Medicago sativa. The yellow precipitate was centrifuged, dried, and collected for further analysis. The characterization carried out via TEM revealed the presence of spherical zinc oxide nanoparticles. Fourier transform infrared (FT-IR) spectroscopy revealed an absorption peak for Zn-O bonding between 400 and 600 cm 1. Additional characterization methods, such as X-ray diffraction (XRD), energy dispersive spectroscopy (EDS) and UV-Vis spectroscopy, confirmed the formation of zinc oxide nanoparticles. An antibacterial test was conducted on the synthesized nanoparticles. This study highlights DPJ as an eco-friendly, cost-effective and novel alternative for the biosynthesis of zinc oxide nanoparticles.