Assessment of Antimicrobial Efficacy of Gentamicin-Treated Cryopreserved Amniotic Membrane in an In Vitro Model

Abstract

Chronic wound infections represent a major global healthcare burden, particularly in patients with diabetes mellitus. Diabetic foot ulcers (DFUs) are frequently complicated by bacterial colonization and biofilm formation, contributing significantly to delayed healing, limb amputation, and increased mortality. In India, the prevalence of diabetic foot complications ranges between 4–10% among diabetic patients, with infection being a leading cause of hospitalization and lower-limb amputation.¹,² Among the causative organisms, Pseudomonas aeruginosa is a clinically important Gram-negative opportunistic pathogen frequently isolated from chronic and burn wounds and is associated with intrinsic resistance mechanisms and biofilm formation.