Ichthyophthiriasis is caused by the parasite Ichthyophthirius multifiliis (Ich). Certain chemical drugs are used as treatments for this parasite. However, given the myriad of detrimental impacts that chemicals have on the environment and people, it is crucial to conduct in-depth research on the efficacy of plant extracts in treating this parasite. This investigation assessed a mixed extract of thyme, mint and ginger in vitro antiparasitic activity against the parasite I. multifiliis. The anti-parasitic activity of solution composed of thyme, mint, and ginger extractagainst I. multifiliis was assessed in a lab setting following a 180-minute exposure at dosages ranging from 0.06 to 8 mL/l. A statistical comparison was made between the obtained data and the outcomes of the negative control treatment and the positive control sample (15 ppm formaldehyde). The dose of 8 and 4 mL/liter corresponds with the lowest time to eradicate more than 80% of parasites. This concentration outperformed formaldehyde, destroyed all of the theronts after 45 minutes, and showed a notable difference from formaldehyde. Higher doses and longer exposure times of the mixed extract can decrease the number of ICH theronts and are appropriate for controlling Ichthyophthiriasis.