[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Indexing and Abstracting::
Articles archive::
For Authors::
For Reviewers::
Registration::
Contact us::
Site Facilities::
Publication Ethics::
Articles in Press::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
:: Volume 2, Issue 2 (11-2022) ::
injoere 2022, 2(2): 16-28 Back to browse issues page
Genome-wide assessment and characterization of simple sequence repeats (SSRs) makers for Capoeta aculeata (Valenciennes, 1844) using NGS data
H. Gandomkar , S. Nazari * , P. Hosseini , H.A. Abdolhay
Abstract:   (1548 Views)
The species Capoeta aculeata (Valenciennes, 1844) is one of the most important freshwater species endemics to Iran. However, the investigation of a population genetic structure of this species is limited by the low number of molecular markers currently described. In this study, we implemented next generation sequencing technology to identify polymorphic microsatellite markers and investigate the population genetic structure of C. aculeata sampled from three geographical sites in Iran. We sequenced 60 individuals from three populations occurring in the Zagros basin. We characterized and developed 36 novel polymorphic microsatellite markers and these loci were examined in 120 individuals from three populations occurring in the Zagros basin. The average number of alleles per locus varied from 1.7 to 16.  (average=7.89). The results showed that, the polymorphism information content (PIC) of these SSR loci varied from 0.254 to 0.888. The observed heterozygosity (Hoper locus ranged from 0.170 to 0.881, while the expected heterozygosity (He) per locus was from 0.170 to 0.881. Among these SSR loci, 20 loci deviated significantly from the Hardy–Weinberg equilibrium after Bonferroni correction (p<0.05). These microsatellite markers could provide a valuable tool for future population and conservation genetics studies of C. aculeate populations and other closely related species.
 
Keywords: Capoeta aculeate, Microsatellite markers, Next generation sequencing (NGS), Genetic structure
Full-Text [PDF 387 kb]   (569 Downloads)    
Type of Study: Research | Subject: General
Received: 2022/05/9 | Accepted: 2022/04/30 | Published: 2022/04/30
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA



XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Gandomkar H, Nazari S, Hosseini P, Abdolhay H. Genome-wide assessment and characterization of simple sequence repeats (SSRs) makers for Capoeta aculeata (Valenciennes, 1844) using NGS data. injoere 2022; 2 (2) :16-28
URL: http://injoere.com/article-1-53-en.html


Rights and permissions
Creative Commons LicenseThis work is licensed under a Creative Commons attributions (CC-BY).
Volume 2, Issue 2 (11-2022) Back to browse issues page
International Journal of Aquatic Research and Environmental Studies
Persian site map - English site map - Created in 0.05 seconds with 36 queries by YEKTAWEB 4645